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Purposeful validation of sorted fractions was carried out by xenotransplantation, the results of which was coupled with miRNA expression profiling to deliver stem cell-related miRNA expression profiles.
To find out irrespective of whether miRNA are differentially expressed in LSC and HSC, we fractionated 16 AML affected individual samples and three lineage-depleted (Lin–) twine blood (CB) samples using CD34 and CD38 into 4 populations and subjected Just about every to worldwide miRNA profiling; the stem mobile material of each and every portion was functionally assayed by xenotransplantation (Figures 1A and S1A).
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(J) Proliferation measured by BrdU incorporation assay of CTRL or 126KD transduced 8227 cells in vitro.
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Self-renewal regulators have remarkably parallel features in malignant and normal stem cells, precluding their therapeutic targeting as a consequence of toxicity to regular stem cells. The opposing self-renewal outcomes governed by miR-126 in HSC and LSC suggest that Regardless of shared stemness determinants, it might be doable to focus on therapeutically the networks that precisely Manage LSC by way of perturbation of miR-126 amounts.
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(E) % BrdU incorporation into bulk cultures showing proliferation of CTRL and 126OE transduced 8227 cells with time.
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